Supplementary Figure 2: Themis2 does not affect B cell development but has a cell intrinsic effect on IgM BCR expression. | Nature Immunology

Supplementary Figure 2: Themis2 does not affect B cell development but has a cell intrinsic effect on IgM BCR expression.

From: Themis2 lowers the threshold for B cell activation during positive selection

Supplementary Figure 2

(a) Number of B cells in wild-type (WT) and Themis2-/- mice, gated on pro/pre (B220+CD43+), immature (B220+CD43-) and mature (B220++CD43-) B cells in the BM; and B1 (FSChighB220lowIgM++) B cells in the peritoneum, including B1a (CD5+) and B1b (CD5-) subsets. Columns show means and s.e.m. (b) The relative proportion of B cell subsets in lethally irradiated mice reconstituted for 8 weeks with 50:50 mixtures of wild-type or Themis2-/- CD45.2 and wildtype CD45.1 BM (gated as in Fig 2). Filled columns show mean percentage CD45.2+ cells and s.e.m., and data are representative of 3 experiments. (c) The mean IgM expression on mature (B220+IgD+) CD45.1 WT and CD45.2 Themis2-/- B cells in chimeric BM, spleen and mesenteric lymph node (MLN). Bars show means and s.e.m..

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