Supplementary Figure 2: UTX is required for development of iNKT cells but not for their function. | Nature Immunology

Supplementary Figure 2: UTX is required for development of iNKT cells but not for their function.

From: The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells

Supplementary Figure 2

(a) Conditional UTX-deficient (UTX KO) mice or wild-type (WT) littermates were injected intraperitoneally with 2 μg α-GalCer. Two hours later, lymphocytes from thymus and liver were collected for intracellular staining of IFN-γ and IL-4, prior to analysis by flow cytometry. Additionally, thymic iNKT cells were restimulated with PMA and Ionomycin for 4 hours before analysis. (b) Flow cytometry analysis of WT or UTX KO thymic iNKT cells by measurement of transcription factors T-bet, PLZF, and RORγt, gated on CD1d-tetramer+ cells. Shown are the absolute cell counts of NKT1, NKT2, or NKT17 cells among thymic iNKT cells from WT or UTX KO (n = 3). (c) Analysis of CD1d expression on DP thymocytes from WT or UTX KO mice using flow cytometry. (d) DP thymocytes were isolated from WT or UTX KO mice. Subsequently, mRNA transcripts of Vα14-Jα18 TCR were analyzed by qRT-PCR and normalized to constant alpha chain (Cα) amplification. Y-axis depicts relative expression (n = 5). Results in (b-d) are representative of three independent experiments. Data are mean ± s.e.m. **P < 0.01 as analyzed by unpaired t-test.

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