Supplementary Figure 6: Cluster analysis of the chromatin landscape around gene promoters in control or UTX-KO iNKT cells.

Heat maps represent the abundance of H3K4me3 and H3K27me3 marks around the promoters of downregulated (a) or upregulated genes (b) in UTX-deficient (KO) iNKT cells compared to wild-type (WT). Genes are subdivided into different clusters dependent on their histone mark pattern. C1-4 = clusters 1 to 4. (c) Integrating gene expression with chromatin state for upregulated genes. Average abundance of H3K27me3 and H3K4me3 around upregulated gene promoters is depicted as z-score in reads per million (rpm). Average profiles are grouped in three clusters of upregulated genes with WT in blue and KO in green. The upper panel shows expression of upregulated genes as logarithmic fold change (log₂FC). (d) GREAT analysis of downregulated genes in UTX-deficient iNKT cells in cluster 3 and cluster 4.