Supplementary Figure 7: Intrinsic function of UTX is responsible for development of iNKT cells.

Mixed bone marrow-chimeric mice were generated using a 1:1 ratio of wild-type (WT) and conditional UTX-deficient (KO) bone marrow cells for transfer into Rag2^-/- hosts (n = 5). Twelve weeks later, WT cells were detected by anti-CD45.1, and frequencies of thymic iNKT cells were analyzed by flow cytometry. (a) Depicted are the ratios of WT to KO iNKT cells gated on the different maturational stages. (b) Bar graph depicting the results from (a). (c) In the same experiment as described in (a), lymphocytes from liver were analyzed for iNKT cells (CD3⁺tetramer⁺) as well as conventional T cells (CD3⁺tetramer^-). Depicted are the ratios of WT to KO T lymphocytes. Results are representative of two independent experiments.