Supplementary Figure 1: Generation of UTX-KO and JMJD3-KO mice. | Nature Immunology

Supplementary Figure 1: Generation of UTX-KO and JMJD3-KO mice.

From: The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells

Supplementary Figure 1

(a) Utx locus in UTX-targeted embryonic stem cells (ESCs) that were provided from EUCOMM. (b) PCR analysis verifying loxP sites targeting exon 3 of Utx in ESC clones. (c) Targeting strategy for Jmjd3 locus to delete exons 17-19. (d) Southern blot analysis of Jmjd3-targeted ESC clones. (e) Representative genotyping of conditional UTX-or JMJD3-KO mice. (f) Analysis of Utx or Jmjd3 mRNA levels in blood, DP thymocytes, or iNKT cells by qRT-PCR using deletion-specific primers. Data are mean ± s.d. from three independent experiments.

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