Supplementary Figure 4: Regulation of proliferation, self-renewal, and apoptosis by TSC2 in macrophages. | Nature Immunology

Supplementary Figure 4: Regulation of proliferation, self-renewal, and apoptosis by TSC2 in macrophages.

From: Chronic signaling via the metabolic checkpoint kinase mTORC1 induces macrophage granuloma formation and marks sarcoidosis progression

Supplementary Figure 4

(a) Images of Tsc2fl/fl and Tsc2fl/fl,Lyz2-Cre BMDM on day 7. Scale bar, 100 μm. (b) Cell cycle analysis of growth-factor deprived Tsc2fl/fl and Tsc2fl/fl,Lyz2-Cre BMDM on day 7. Shown are means ± s.e.m. (n= 3). (c) IHC for Ki-67 in a paw section of a 6 month-old Tsc2fl/fl,Lyz2-Cre mice. Scale bar, 200 μm. (d) Cell cycle analysis of CSF1-stimulated BMDM treated with solvent or 100 nM rapamycin for 18 hours. Shown are means ± s.e.m. (n= 3). (e) Whole cell lysates of Tsc2fl/fl and Tsc2fl/fl,Lyz2-Cre BMDM were analyzed by immunoblotting with the indicated antibodies. (f) GSEA of the 'Self-renewal' gene signature (WONG_EMBRYONIC_STEM_CELL_CORE) in Tsc2fl/fl,Lyz2-Cre BMDM relative to Tsc2fl/fl BMDM. Data are from one experiment with four biological replicates per genotype. (g) IHC for survivin in lung sections of 3 month-old Tsc2fl/fl and Tsc2fl/fl,Lyz2-Cre mice. Scale bar, 100 μm. Results are representative of three mice per genotype (a,c,g), cumulative for three (b,d) mice per genotype, or are from one experiment with two (e) or four biological replicates per genotype (f).

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