Supplementary Figure 2: Identification and characterization of the hypertrophic macrophages in the lungs of Tsc2fl/fl,Lyz2-Cre mice. | Nature Immunology

Supplementary Figure 2: Identification and characterization of the hypertrophic macrophages in the lungs of Tsc2fl/fl,Lyz2-Cre mice.

From: Chronic signaling via the metabolic checkpoint kinase mTORC1 induces macrophage granuloma formation and marks sarcoidosis progression

Supplementary Figure 2

(a) Flow cytometry plots of lung single cell suspensions at 10 weeks. The hypertrophic macrophages are indicated as “hypertroph. MΦ”. (b) Flow cytometric analysis of the indicated surface markers of alveolar macrophages, interstitial macrophages, and Ly6Chi monocytes in Tsc2fl/fl and Tsc2fl/fl,Lyz2-Cre mice and their comparison to the hypertrophic macrophages in the lung of the Tsc2fl/fl,Lyz2-Cre mice. (c) Flow cytometric analysis of immune cell populations in the lung of 10 week-old Tsc2fl/fl and Tsc2fl/fl,Lyz2-Cre mice. Shown are means ± s.d. (d) Bone marrow-derived macrophages were unstimulated or stimulated with either 100 ng/ml LPS and 20 ng/ml IFNγ or with 10 ng/ml IL-4 for 48h. Expression of the indicated mRNAs normalized to β-actin is shown as means ± s.e.m. Data are representative of five mice (a,b) per genotype or cumulative of five (c,d) individual mice per genotype.

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