Supplementary Figure 5: Sestrin inhibition enhances antigen-specific functions in old T cells.
From: A sestrin-dependent Erk–Jnk–p38 MAPK activation complex inhibits immunity during aging
Senescent CD4+ T cells from old humans (70-85 years) were transduced as indicated, rested for 10 days, then challenged with autologous antigen presenting cells (APCs) pre-oaded with the indicated CMV antigen dilutions. After additional 3 days (a) proliferation and (b) cytokine production was examined by flow-cytometry (n = 3). Antigen-specific proliferation data presented relative to those of cells transduced with shCtrl, and stimulated with the lowest CMV antigen dilution (1:50), set as 1.
