Supplementary Figure 7: In vivo MAPKi suppresses immune function in sestrin-negative cells.
From: A sestrin-dependent Erk–Jnk–p38 MAPK activation complex inhibits immunity during aging
(a) Phospho-flow of Erk, Jnk and p38 phosphorylation in CD4+ T cells from control Sesn1+/- and Sesn1-/- null mice 5 days after vaccination with FLUAD. Data presented relative to those of control Sesn1+/-, set as 1. (b) Spleens of 16-month old mice vaccinated with FLAUD and daily kept on Erk, Jnk and p38 MAPK inhibitors or all three inhibitors together (triple MAPKi). DMSO, vehicle control. (c) Impact of MAPKi on immune-responsiveness of sestrin2- CD4+ T and CD19+ B cells to vaccination with FLAUD in vivo, presented relative to DMSO-injected (control) mice, set as 1 (n = 4). ***p< 0.001, ANOVA with Bonferroni post-test correction. Error bars depict s.e.m.
