Supplementary Figure 6: Depletion of IL-4 ‘rescues’ Foxp3 expression in MSC-deficient iT_reg cells.

(a) Activated WT naive CD4⁺ T cells were stimulated with combinations of TGF-β with indicated dosage of anti-IL-4 antibody for 3 days. The frequency of Foxp3⁺ cells was determined by flow cytometry; (b) Quantification of the frequency of Foxp3⁺ iT_regs as in (a); (c) Flow cytometry analysis and (d) Quantification of Foxp3⁺ T_regs in CD4⁺ T cells in the thymus from indicated mice; (e) Quantification of Foxp3⁺Nrp1^– iT_regs or Foxp3⁺Nrp1⁺ tT_regs from dLN as in Fig 5d; (f) Quantification of Foxp3⁺Nrp1^– iT_regs or Foxp3⁺Nrp1⁺ tT_regs from LP as in Fig 5d; The expression of Msc was analyzed by q-PCR. Data are representative of three independent experiments (a, c) with n = 4 mice each group or are pooled from three independent experiments (b, d-f). *P < 0.05, (Student's t-test, error bars, SD).