Supplementary Figure 7: MSC interacts with GATA3 in iTreg cells. | Nature Immunology

Supplementary Figure 7: MSC interacts with GATA3 in iTreg cells.

From: The transcription factor musculin promotes the unidirectional development of peripheral Treg cells by suppressing the TH2 transcriptional program

Supplementary Figure 7

(a) GATA3 and MSC association was visualized in different T cell subsets with an in situ proximity ligation assay. Punctate staining (red) indicates a GATA3-MSC interaction as detected by the assay; (b) Co-immunoprecipitation of GATA3 and MSC from extracts of co-transfected 293T cells with or without ethidium bromide (EtBr). HEK293T cells were transfected with mouse Flag-tagged GATA3 and V5-tagged MSC. Anti-V5 immunoprecipitates (IP) and total lysates were immunoblotted with anti-V5 and anti-Flag antibodies; (c) Co-immunoprecipitation of GATA3 and MSC from extracts of in vitro differentiated iTregs with or without ethidium bromide (EtBr). Anti-GATA3 immunoprecipitates (IP) and total lysates were immunoblotted with anti-GATA3 and anti-MSC antibodies. Data are representative of three independent experiments.

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