Supplementary Figure 8: MSC promotes the suppressive effect of iT_reg cells on the T_H2 response.

(a) q-PCR analysis of indicated co-inhibitory molecules in GFP⁺ (Foxp3⁺) WT and Msc^–/– iT_regs; (b) Naive CD45.1⁺CD4⁺ T cells were cocultured with CD45.2⁺Foxp3⁺ WT and Msc^–/– iT_regs under differentiation conditions for T_H1, T_H2, and T_H17 cells. Intracellular staining of indicated cytokines produced by CD45.1⁺ different polarized T cell subsets cells was determined by flow cytometry; (c) Flow cytometry analysis of intracellular cytokines in gated CD4⁺ T cells from the lung of HDM administrated mice with indicated treatments. Data are representative of three independent experiments (a, b, c) with n = 4 mice each group. *P < 0.05, **P < 0.01, (Student's t-test, error bars, SD).