Supplementary Figure 8: Increased IgM production in chimeras reconstituted with Fcmr–/– B-1 cells and wild-type BM cells. | Nature Immunology

Supplementary Figure 8: Increased IgM production in chimeras reconstituted with Fcmr–/– B-1 cells and wild-type BM cells.

From: The IgM receptor FcμR limits tonic BCR signaling by regulating expression of the IgM BCR

Supplementary Figure 8

For lethally-irradiated B-1 chimeras, 2-months old μMT mice were lethally irradiated one day before transfer of 5 x 106 peritoneal lavage cells from Fcmr flx/flxCd19-Cre or control mice (IgHb) as a source of B-1 cells and BM cells from wild-type (IgHa) mice as a source of B-2 cells. Chimeras were rested for at least 7 weeks before analysis (n=4 mice/group). (a) Graphs summarize mean frequencies ± SD of IgMb+ secreting cells derived from B-1 cells and IgMa+ secreting cells derived form BM B-2 cells in spleens, and BMs as assessed by ELISPOT analysis. (b) Mean IgMb, IgMa, and IgM concentrations ± SD in sera of the chimeras, measured by ELISA. *p<0.05, **p<0.005, ***p<0.0005 by unpaired two-tailed Student’s t-test.

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