Supplementary Figure 1: FcμR is expressed by various cell subsets, including peripheral B cells.
From: The IgM receptor FcμR limits tonic BCR signaling by regulating expression of the IgM BCR
(a) Overlay histograms comparing surface FcμR expression in B cells, CD4 T cells, CD8 T cells, Gr-1+ (Granulocytes); CD11b+ and F4/80+ (Macrophages); CD11c+ (Dendritic Cells) from Fcmr flx/flxCd19-Cre and controls. (b) 5% contour plots with outliers of representative spleen samples from wild-type mice analyzed by flow cytometry after gating on live non-doublets. Gating shows FcμR+ of indicated cell subsets. Numbers in black indicate frequencies of FcμR+ cells among total live cells, and those in blue indicate frequencies of FcμR+ cells among CD19+, CD4+, CD8+, Gr-1+, CD11b+, and CD11c+ cells, respectively. Small contour plots are FMOs. (c) Overlay histograms show surface FcμR expression in different B cell subsets in spleen, peritoneal cavities (perc), and inguinal lymph nodes (pLN): spleen marginal zone (MZ), spleen follicular B (FO), spleen B-1, peritoneal cavity (perc) B-1, perc B-2, and peripheral lymph node (pLN) FO B cells. (d) Overlay histograms comparing surface FcμR expression in B cell precursors, including late pre B cells, immature B cells, and mature B cells.
