Supplementary Figure 6: Tr1 BATF-dependent Network (Zoom-in)

RNA-seq and ATAC-seq datasets were integrated to generate putative transcriptional regulatory networks for Batf^−/− Tr1 cells. Nodes represent differentially expressed transcription factors and target genes, colored according to relative gene expression (log2(Batf^−/−/Control)) at 72h; red and blue indicate high and low relative expression, respectively. Edges are drawn between differentially expressed TFs and putative gene targets if that TF’s motif was enriched in ATAC-seq peaks cis to genes that were increased or decreased in response to Batf^−/− (Praw<10-3, hypergeometric CDF). Edge colors (blue - inhibitory, red - activating) are based on correlation between TF and putative target gene expression. “ON” indicates genes de-repressed in the knockout cells, while “OFF” indicates genes repressed in the knockout cells.