Supplementary Figure 3: Nlrp12 defieciency causes significant changes in the intestinal microbiota.

(a) Schematic showing fecal DNA collection from mice housed in two distinct vivarium for 16s rRNA gene sequencing. (b) Bacterial diversity within WT (n=18) and Nlrp12^–/– (n=15) mice, (c) PCoA plot showing microbiota compositional differences between WT and Nlrp12^-/- mice from a repeated 16S rRNA gene microbiome sequencing experiment conducted in vivarium #2. (d) Schematic showing fecal DNA collection from Nlrp12^+/+ and Nlrp12^–/– littermates derived from the same parents for 16s rRNA gene sequencing. (e) PCoA plots of microbiota compositional differences between Nlrp12^+/+ and Nlrp12^–/–littermates. Error bars show SEM. One dot represents one mouse. **p<0.01 determined by two-tailed unpaired t test.