Supplementary Figure 4: Nlrp12^–/– mice cohoused with wild-type mice display attenuated colitis.

(a) Representative images of H&E-stained colons from DSS-treated cohoused or single-housed WT and Nlrp12^–/– mice. (b) Body weight, (c) percent survival (^† indicates statistical significance between SiHo WT vs. SiHo Asc^–/–, and * indicates significance between SiHo WT vs. CoHo WT), (d) DAI and (e) colon length of DSS-treated cohoused or single-housed WT and Asc^–/– mice (CoHo WT and CoHo Asc^–/–, n=4; SiHo WT and SiHo Asc^–/–, n=6). (f) Representative images of H&E-stained colons of SiHo and Coho WT and Asc^–/– mice after DSS-induced colitis. (g) PCoA plot showing fecal microbial composition before cohousing (n=9/group). (h) Quantification of UniFrac distance between mice from (g) after cohousing, indicated as dissimilarity values. Error bars show SEM. *p<0.05, ***p<0.001 and n.s. means no significance, determined by two-tailed unpaired t test (b, d, e), Log-rank (Mantel Cox) test (c) and ANOSIM (h). Scale bars represent 1 mm for 40X and 200 μm for 200X (a,f). The Tukey’s boxplot indicates first (bottom of the box) and third quartiles (top of the box), and the band inside the box is the median, and the ends of the whiskers indicate 1.5 interquartile range of the upper or lower quartile (h).