Supplementary Figure 3: Proteome differences and marker proteins.
From: Social network architecture of human immune cells unveiled by quantitative proteomics
(a) Proteome comparison of naive CD4 with naive CD8 T cells (Welch’s t-test, FDR<5%, S0=1). Significant proteins are marked in blue containing the lineage markers CD4 and CD8A.
(b) Proteome comparison of naive CD4 with classical monocytes, respectively (Welch’s t-test, FDR<5%, S0=1). Significant proteins are marked in blue and the top 50 differentially abundant proteins are named.
(c) Number of significantly (Welch’s t-test, FDR<5%, S0=1) different proteins by pairwise proteome comparison (NOT: steady state, ACT: activated).
(d) Expression comparison (z-scored MaxLFQ values) of marker proteins for B cell plasma blasts (top) and for CD8 TEMRA cells (bottom). Potential unique marker proteins are indicated below the grey horizontal line. For clarity z-score values of other cell lineages were removed except for cell lineage markers (CD19, CD8B) and potential exclusive cell type markers. The isoforms of CD45 could not be resolved from the proteome analysis, therefore CD45RO and CD45RA are not included in the plot.
(e) Median fluorescence intensity (MFI) of PLVAP (intracellular staining) in B cells determined by flow cytometry and normalized by isotype control MFI (n=2, two independent donors, left). Representative histograms of fluorescence intensities of isotype control and PLVAP (right).
