Supplementary Figure 7: Ablation of Nr3c1 cofactor Ncor1 and treatment with dexamethasone affect the differentiation of MP CD8⁺ T cells.

(a) Schematic view of experimental design. OT-I CD8⁺ T cells were activated in vitro and transduced with control shRNA or shYy1 retroviral vectors and subsequently co-transferred into recipient mice followed by i.v. infection with Lm-OVA. Splenocytes were isolated and analyzed on day 7 of infection. (b) Flow cytometric analysis of KLRG1 and IL-7R expression for cells transduced with shCd4 and shNcor1 in PBL on day 8 of infection. (c) The percentage of TE and MP CD8⁺ T cells gated on transduced cells on day 8 of infection after knockdown of Ncor1. (d) Flow cytometric analysis of KLRG1 and IL-7R expression for donor cells in mice treated with either vehicle or dexamethasone for 7 days. (e) The percentage of TE and MP CD8⁺ T cells gated on donor cells on day 8 of Lm-OVA infection after drug treatment. Data are representative from two independent experiments with 5 mice per group. The statistical analysis was performed by two-tailed paired t-test in (c) and two-tailed unpaired t-test in (e). *: p<0.001