Supplementary Figure 1: Transcriptional program of the TE and MP CD8⁺ T cell subsets.

(a) Comparison of gene expression of TE and MP CD8⁺ T cell subsets by microarray. Genes that are 1.5-fold upregulated in TE or MP CD8⁺ T cell subsets are highlighted as blue or orange, respectively. Transcripts did not differ significantly in expression following correction with FDR therefore a fold-change cut-off of 1.5-fold was used for comparisons.(b) Volcano plots of the comparison of total effector and memory CD8⁺ T cells highlighting TE- or MP-enriched genes. Numbers in bottom corners indicate the number of highlighted genes in that region. (c) The ratio of gene expression of known TFs in TE versus MP subset from microarray. (d) Histograms of protein abundance of key TFs in TE versus MP subset. (e) Comparison of gene expression of TE and MP CD8⁺ T cell subsets by RNA-seq. Genes that are 2-fold upregulated in TE or MP CD8⁺ T cell subsets are highlighted as blue or orange, respectively. (f) GSEA plot of total effector versus memory CD8⁺ T cells with TE- or MP-enriched gene signature generated from (e). (g) The ratio of gene expression of known TFs in TE versus MP subset from RNA-seq. Data are the mean value of gene expression from three independent experiments with pooled spleens from three mice except the TE subset in (a) which is from two independent experiments. Data in (d) are representative of two independent experiments with three mice per group.