Supplementary Figure 5: The interaction of IRAP with FHOD4 can be reconstituted in wild-type fibroblasts.
From: IRAP+ endosomes restrict TLR9 activation and signaling
(a) WT fibroblasts were transfected by electroporation with a plasmid expressing FHOD4-GFP. Thirty-six hours later IRAP and FHOD4 were immunoprecipitated with anti-IRAP and anti-GFP respectively and the precipitates were split in two and analyzed by immunoblot as indicated. (b) WT and KO fibroblasts were transfected as in (a) and a proximity ligation assay for detection of IRAP/FHOD4 interaction was performed with antibodies against IRAP and GFP.
