Supplementary Figure 4: Deletion of Sabt1 or Bptf leads to reduced numbers of ILC3s.
From: Long noncoding RNA lncKdm2b is required for ILC3 maintenance by initiation of Zfp292 expression
(a,b) Percentages (a) and numbers (b) of ILC3s in small intestines derived from Satb1+/+ and Satb1-/- mice. Percentages of indicated cells were calculated and shown at right panel (a). n=6 per group. (c,d) Percentages (c) and numbers (d) of ILC3s in small intestines derived from Bptfflox/flox and Bptfflox/floxRorc-Cre mice. Percentages of indicated cells were calculated and shown at right panel (c). n=6 per group. (e) Bptfflox/flox and Bptfflox/floxRorc-Cre mice were infected with C. rodentium (5x109 CFUs per mouse). 6 days later, bacterial counts (CFUs) in feces were measured. n=6 for each group. (f) Bptfflox/floxRorc-Cre− and Bptfflox/floxRorc-Cre+ mice were infection with C. rodentium (5x109 CFUs per mouse) for 6 days, followed by H&E staining for colons. Scale bar, 50 μm. (g) 1x106 CD45.1 BM cells with 1x106 CD45.2 LPLs from WT or TKO mice infected with Zfp292 expressing retroviral were transplanted into lethally irradiated CD45.1 recipients. Percentages of ILC3s derived from CD45.2+ donors were analyzed by FACS. n=6 for each group. (h) Numbers of ILC3s as in g were analyzed by FACS. n=6 for each group. (i) Percentages of Ki67+ ILC3s in Zfp292+/+, lncKdm2b overexpression in Zfp292+/+ or Zfp292-/- LPLs were examined by FACS. n=6 for each group. *p<0.01 and **p<0.001 by two-tailed Student’s t test. All data are representative of at least three independent experiments.
