Supplementary Figure 3: Sabt1 interacts with the NURF complex.
From: Long noncoding RNA lncKdm2b is required for ILC3 maintenance by initiation of Zfp292 expression
(a) Identification of Bptf and Snf2l as Satb1 binding proteins. Bptf and Snf2l protein sequences were identified by LTQ Orbitrap XL. (b) Generation of Zfp292-/- mice. The schematic diagram of Satb1 KO construction by CRISPR/Cas9 technology (upper left). Frameshift of Zfp292 gene was confirmed by DNA sequencing (lower left). Zfp292 deletion was verified by immunoblotting (right panel). (c) LPLs from Zfp292+/+ and Zfp292-/- mice were isolated and stimulated with IL-23 for 4 h. Numbers of IL-22+ ILC3s were examined by FACS. *p<0.01 by two-tailed Student’s t test. All data are representative of three independent experiments.
