Supplementary Figure 3: Binding of STAT5, PAX5, EBF, PU.1, IRF4, and IKAROS at genes that govern pre-B cell transcriptional networks

(a) Occupancy of STAT5, PAX5, EBF, PU.1, IRF4 and IKAROS by ChIP-Seq at Il7r, Jak1, Stat5b, Stat5a and Socs3 loci in Stat5b-CA x Blnk^+/- leukemia (STAT5), Rag^-/- pro-B cells (PAX5, EBF, PU.1, IRF4) or WT pre-B cells (IKAROS). Loci are annotated for progenitor B cell super-enhancers (SE). (b) Occupancy of STAT5, PAX5, EBF, PU.1, IRF4 and IKAROS by ChIP-Seq at Pax5, Ebf1, Sfpi, Irf4 and Ikzf1 loci. Loci are annotated for progenitor B cell super-enhancers (SE). Data are representative of one (a, b (STAT5, PAX5, EBF, PU.1, IRF4) or two (a, b, IKAROS) independent experiments. Data for PEPII ChIP-Seq experiments in d,c came from^30,36-38. Defined super-enhancers in c came from³⁹.