Supplementary Figure 1: Mouse B-ALL-like leukemias most closely resemble large pre-B cells by global mRNA expression pattern and have deregulated expression of several tumor suppressors and oncogenes
From: Antagonism of B cell enhancer networks by STAT5 drives leukemia and poor patient survival
(a) Spearman correlation coefficients calculated between expression data from WT pre-B cells (n = 5 samples pooled from 3-8 mice), Xid pre-B cells (n = 3 samples pooled from 3-8 mice), Stat5b-CA pre-B cells (n = 4 samples pooled from 3-8 mice), Stat5b-CA leukemias (n = 6 mice), Stat5b-CA x Blnk+/– leukemias (n = 5 mice), Stat5b-CA x Xid leukemias (n = 5 mice), or Stat5b-CA x Prkcb–/– leukemias (n = 4 mice) and the Hardy fractions of B cell development from immgen.org. (b) Quantitative real-time PCR for Bach2, Ikzf1, Myc, and Socs2 expression in WT pre-B cells or Stat5b-CA x Blnk+/–, Stat5b-CA x Xid, and Stat5b-CA x Prkcb–/– leukemias. Note: Ikzf1 and Myc are known NF-κB target genes. Black lines indicate means (b). *P < 0.05 **P < 0.01 (Unpaired t-test, b). Data are representative of three (WT) or four (Stat5b-CA x Blnk+/–, Stat5b-CA x Xid, and Stat5b-CA x Prkcb–/–) independent experiments (b).
