Supplementary Figure 3: Crown like structures (CLSs), non–CLS-associated macrophages and macrophage–adipocyte contact area in the SAT of obese Cre+α4f/f and Cre−α4f/f mice.
From: A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
a ) Representative sections demonstrating macrophage staining (F4/80 staining) in SAT from obese Cre+α4f/f and Cre−α4f/f mice are shown. Scale bar is 100μm. b) Quantification of CLS and non-CLS-associated macrophages in SAT from obese Cre+α4f/f (n=5 mice) and Cre−α4f/f (n=5 mice) mice is depicted. Shown are the number of CLS per 100 mm2 of tissue and the number of non-CLS-associated macrophages per 100 adipocytes. c) The surface area of macrophages in contact with adipocytes from the SAT of obese Cre+α4f/f (n=5 mice) and Cre−α4f/f (n=5 mice) mice was calculated. Data are expressed as μm2 of contact area per macrophage.
Data are presented as mean ± SEM. *P < 0.05 Student's t-test in (b), Mann-Whitney U in (c). In (a) representative histological images are from analysis performed on 5 mice per genotype. Data in (b)-(c) are from one experiment.
