Supplementary Figure 4: α4-integrin-dependent adhesive interactions between macrophages and adipocytes in the obese SAT.
From: A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
Immunofluorescence analysis for macrophages (F4/80, green), adipocytes (caveolin-1, red) and DAPI (blue) was performed in the SAT of obese Cre−α4f/f and obese Cre+α4f/f mice. (a) Representative 3D-reconstruction of a macrophage-adipocyte interaction in the SAT of an obese Cre−α4f/f mouse is shown. The white line shows the position of the sectional plane depicted in (b). Panel (b) depicts a 70μm long segment of the sectional plane shown in (a) containing the macrophage-adipocyte contact area. (c) Representative 3D-reconstruction of a macrophage-adipocyte interaction in the SAT of an obese Cre+α4f/f mouse is shown. The white line shows the position of the sectional plane depicted in (d). Panel (d) depicts a 70μm long segment of the sectional plane shown in (c) containing the macrophage-adipocyte contact area. Representative images are from analysis performed on 5 mice per genotype.
