Supplementary Figure 4: Loss of Nrros from resident CNS macrophages but not peripheral myeloid cells causes neurological disorders.
From: Mice deficient in NRROS show abnormal microglial development and neurological disorders
(a - b) RT-qPCR analysis for Nrros expression in CD11b+ cells isolated from the brains of mice of the indicated genotype. Performance of (c - g) Nrrosfl/flVav-cre or (h - l) Nrrosfl/flLyz2-cre mice in rotarod test at (c, h) pretraining (9-week old), (d, i) fixed and (e, j) accelerating condition. S, session; T, trial. (f, k) Total beam breaks and (g, l) percent center beam breaks at open field tests. Animals were 10-11-week old unless specified otherwise. N = 16. (m-o) Nrros+/+ (WT) and Nrros-/- (KO) mice were sub lethally irradiated and reconstituted with donor marrow from either WT or KO mice (n = 15, 18, 14, 10). (m) Survival curve of WT and KO recipients grafted with indicated bone marrow stem cells. Quantification of percent engraftment in the blood (n) and brains (o) of these animals (n = 3). Error bar: ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. (m) Log-rank test; all other analyses, unpaired Student’s t-test. ND, not determined.
