Supplementary Figure 3: Smad3 and STAT3 synergistically upregulate Taz expression. | Nature Immunology

Supplementary Figure 3: Smad3 and STAT3 synergistically upregulate Taz expression.

From: The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells

Supplementary Figure 3

Identification of Smad3 and STAT3 by mass spectrometry in streptavidin-precipitation assays using biotinylated Taz gene 1-kb promoter (Taz p1000) DNA fragments in lymphoid tissue lysates of WT mouse. The control DNA was amplified from PGL3-Basic vectors using the same primer set. The DNA gel in the bottom shows the input of biotinylated DNA fragments. M, molecular size markers. The data are representative of three independent experiments with similar results.

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