Supplementary Figure 4: TAZ directly binds to RORγt and enhances its transcriptional activity. | Nature Immunology

Supplementary Figure 4: TAZ directly binds to RORγt and enhances its transcriptional activity.

From: The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells

Supplementary Figure 4

(a) Identification and the list (the right panel) of TEAD3, TEAD4, RORγt, Foxp3 and Tip60 by mass spectrometry in a Flag-tagged TAZ-precipitation assay in lymphoid tissue lysates of WT or Taz Tg mice. *, migration of TAZ; IP, immunoprecipitation; IB, immunoblot analysis; M, molecular size markers. (b, c) Immunoassays of 293T cells expressing various combinations (above lanes) of GFP-tagged RORγt and full-length (FL) or the truncated fragment of Flag-TAZ as indicated; immunoprecipitation with anti-Flag and analysis by immunoblot with the indicated antibodies; below, immunoblot analysis of total cell lysates (TCL) without immunoprecipitation. (d) Immunoassays (as in b, c) of 293T cells expressing various combinations (above lanes) of GFP-tagged TAZ and full-length (FL) or the truncated fragment of Flag-RORγt as indicated. (e) Super-resolution immunofluorescence imaging (SIM) of HeLa cells transfected with nuclear localization sequence (NLS)/Flag-tagged TAZ (red), full-length, DBD, Hinge or LBD fragment of RORγt (green). Scale bars, 20 μm. The data are representative of three independent experiments with similar results (a-e), or with ~50 cells (e)

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