Supplementary Figure 1: Characterization of mice with conditional knockout of MST1/2 or TAZ in T cells.

(a) Flow cytometric analysis (top panel) and quantification (lower panel) of thymocytes from 8-week-old Mst1^f/fMst2^f/f mice or Mst1^f/fMst2^f/f Ox40-Cre mice, stained with anti-CD4 and anti-CD8 antibodies. (b-d) Flow cytometric analysis (b) and quantification of CD3⁺CD4⁺ or CD3⁺CD8⁺ T cell (c), the naïve T cell (CD62L^highCD44^low) and the effector T cell (CD62L^lowCD44^high) (d) populations in the spleen or lymph node from Mst1^f/fMst2^f/f mice and Mst1^f/fMst2^f/f Ox40-Cre mice, with the indicated antibodies. (e and f) Photograph of 10-month-old Mst1^-/- KO mice with Sjögren’s syndrome (e, left panel). Representative H&E-stained eye sections with associated lymphocytic cell infiltration of the lacrimal gland (e, right panel). Relative mRNA levels of Taz, Ifng, Il17a, Il17f and Il23r genes in the lacrimal gland by Real-time quantitative PCR (f). Scale bars, 500μm. The data are representative of three independent experiments with similar results (a-f). The data represent mean ± s.d. (n=3). ns, not significant (P > 0.05); *P < 0.05, **P < 0.01 and ***P < 0.001 compared with control, Student’s t-test.