Supplementary Figure 3: Genomic targeting of Mir31.
From: The microRNA miR-31 inhibits CD8+ T cell function in chronic viral infection
(a) Diagram of conditional miR-31 targeting construct, showing locations of pre-miR-31, inserted LoxP sites, and flpE flanked neomycin resistance cassette. (b) Mir31flox/flox mice were crossed to Zp3cre mice to generate Mir31-/- mice. CD8+ T cells from WT or Mir31-/- mice were not treated (Unstimulated) or stimulated with aCD3/CD28 for 24 hours. Relative miR-31 expression was determined by qPCR. (c) Mir31flox/flox mice were crossed to Cd4cre mice to generate Mir31flox/flox Cd4cre mice. CD8+ T cells from Mir31flox/flox or Mir31flox/flox Cd4cre mice were not treated (Unstimulated) or stimulated with CD3+CD28 antibodies for 24 hours. Relative miR-31 expression was determined by qPCR. Data are representative of 2 (b-c) experiments. Data show mean ± s.d.
