Supplementary Figure 6: LCMV RNA persistence, Foxp3 gene expression and CD4+ Treg cell populations in wild-type and Mir31–/– mice. | Nature Immunology

Supplementary Figure 6: LCMV RNA persistence, Foxp3 gene expression and CD4+ Treg cell populations in wild-type and Mir31–/– mice.

From: The microRNA miR-31 inhibits CD8+ T cell function in chronic viral infection

Supplementary Figure 6

(a) LCMV Gp gene expression relative to Gapdh was quantified by qPCR in total cDNA from liver and kidney of WT and Mir31-/- mice on day 30 following infection with LCMV clone 13 (n= 4, 5 (WT, Mir31-/-), p values: 0.04, 0.02 respectively)). (b) Luciferase reporter assay for reporter genes containing no 3’UTR (control), Ppp6c 3’UTR, or Foxp3 3’UTR in the presence of miR-31. (c) Intracellular staining for Foxp3 expression in CD4+ T cells from blood, spleen, peripheral lymph nodes (PLN) or mesenteric lymph nodes (MLN) from WT and Mir31-/- mice. Data are representative of two experiments.

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