Supplementary Figure 7: Acute anti-viral responses are not altered in Mir31^–/– mice.

Mir31^+/- / and Mir31^-/- mice were intra-nasally infected with 1.6x10⁵ TCID₅₀ of influenza strain X31-GP33. (a) Body weight was measured daily before (day 0) and following infection. Percentage of original body weight is shown. (b) Quantification of influenza RNA by qPCR in total lung RNA on day 8 following infection. (c) Cells from spleen and lung at 8 dpi and 30 dpi were stained with H-2D^b tetramers specific for GP33, NP or PA peptides. Data represent the sum of the three CD8⁺ CD44⁺ tetramer⁺ populations as a fraction of total CD45⁺ cells (n≥4mice per group). Data are representative of one experiment.