Supplementary Figure 2: ACKR1 is expressed by endothelial cells and nucleated erythroid cells in the BM but not by other hematopoietic cells. | Nature Immunology

Supplementary Figure 2: ACKR1 is expressed by endothelial cells and nucleated erythroid cells in the BM but not by other hematopoietic cells.

From: Atypical chemokine receptor 1 on nucleated erythroid cells regulates hematopoiesis

Supplementary Figure 2

(a) Immunofluorescence micrographs of wild-type (WT) and ACKR1-deficient (KO) BM stained with anti-ACKR1 (red), anti-CD31 (endothelial cells, blue), anti-Ter119 (erythroid cells, yellow) antibodies and DAPI (nuclei, turquoise). (b) ACKR1 expression (MFI) on HSPCs, megakaryocyte progenitors (MkP) and leukocytes (Leu) as compared to the subpopulations of erythroid cells at different stages of development (I-VI) in WT (red) and KO (blue) BM (Mean±SEM; n=3). Scale bar, 30 μm (c) Human ACKR1 mRNA BM expression data retrieved from DMAP (http://portals.broadinstitute.org/dmap/home). Subpopulations of erythroid cells (ERY1-4) were defined as follows: CD71hi GlyA–(Ery1), CD71hi GlyA+(Ery2), CD71lo GlyA+(Ery3) and CD71–GlyA+(Ery4). Mean±SEM, One-Way ANOVA; *P < 0.001.

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