Supplementary Figure 4: Flow cytometry analysis of HSPCs, CD48⁺ subpopulations of LSK cells and CLP cells.

(a) Gating strategy to identify HSPC populations. LSK, defined as Lin^–Sca-1⁺c-Kit⁺ were subdivided into LSK CD48⁺ and LSK CD48⁺. Myeloid progenitor cells (MPC) were defined as Lin^–Sca-1^–c-Kit⁺. (b) Frequency of LSK CD48⁺ cells in in WT (blue) and ACKR1-deficient (KO, red) BM. (c) Relative distribution of their MPP2, and lineage-restricted progenitor (LRP) 1 and LRP2 sub-populations. Representative dot plots, left; quantitative analysis, right. (d) Frequency of MPP2, LRP1 and LRP2 in the BM of WT and KO mice. (e) Frequency of common lymphoid progenitors (CLP) defined as Lin^–Sca-1^loc-Kit^lo (G1 gate in a) and IL7Rɑ⁺Ftl3⁺. Representative dot plots, left; quantitative analysis, right. (b-d) n=12 in four independent experiments. (f) n=5 in two independent experiments. All data show Mean±SEM. Two-tailed Student’s t-test; ***p < 0.001.