Supplementary Figure 5: Time-lapse imaging in nanowell grids (TIMING). | Nature Immunology

Supplementary Figure 5: Time-lapse imaging in nanowell grids (TIMING).

From: IgG Fc domains that bind C1q but not effector Fcγ receptors delineate the importance of complement-mediated effector functions

Supplementary Figure 5

Raji tumor cells and NK cells are stained with PKH26 and PKH67 respectively, deposited en masse on a grid containing thousands of nanowells, immersed in fluorescent annexin V-containing medium and imaged for 6 h by high throughput timelapse microscopy. Imaging data were analyzed as described (Merouane Bioinformatics).

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