Supplementary Figure 6: Cytokine production in the supernatant from day 3 co-cultures of OT-II T cells and DCs. | Nature Immunology

Supplementary Figure 6: Cytokine production in the supernatant from day 3 co-cultures of OT-II T cells and DCs.

From: Increased cathepsin S in Prdm1−/− dendritic cells alters the TFH cell repertoire and contributes to lupus

Supplementary Figure 6

DCs were purified from the spleens of female wild-type (open box) or CKO (gray box) mice, and CD4+ T cells were purified from female OT-II mice. DCs and T cells were co-cultured for 3 days at a 1:10 ratio (DC:T) with either OVA323-339 peptide (100 ng/ml) or OVA protein (10 μg/ml). Cytokine levels were measured in the supernatant by multiplex assay. In the box-and-whisker plot, horizontal bars indicate the median, boxes indicate 25th to 75th percentile, and whiskers indicate 10th and 90th percentile (n=6).

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