Supplementary Figure 7: Proliferation and IL-21 production of OT-II T cells by DCs presenting OVA peptide (amino acids 323–339). | Nature Immunology

Supplementary Figure 7: Proliferation and IL-21 production of OT-II T cells by DCs presenting OVA peptide (amino acids 323–339).

From: Increased cathepsin S in Prdm1−/− dendritic cells alters the TFH cell repertoire and contributes to lupus

Supplementary Figure 7

OT-II T cells were co-cultured with splenic DCs for 3 days at 1:10 ratio (DC:T cells) in the presence of OVA323-339 peptide. To assess proliferation, T cells were labeled with CFSE (10 mM) before the culture and proliferation was measured by calculation of the division index (left panel). In the box-and-whisker plot, horizontal bars indicate the median, boxes indicate 25th to 75th percentile, and whiskers indicate 10th and 90th percentile (n=3). To assess IL-21 production, culture supernatant was harvested and measured by Multiplex assay (right panel). In the box-and-whisker plot, horizontal bars indicate the median, boxes indicate 25th to 75th percentile, and whiskers indicate 10th and 90th percentile (n=3).

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