Supplementary Figure 8: Protein level of cytokines, IL-2 and IL-21, measured by intracellular staining of T cells. | Nature Immunology

Supplementary Figure 8: Protein level of cytokines, IL-2 and IL-21, measured by intracellular staining of T cells.

From: Increased cathepsin S in Prdm1−/− dendritic cells alters the TFH cell repertoire and contributes to lupus

Supplementary Figure 8

OT-II T cells were co-cultured with splenic DCs that had been incubated with HEL (10 μ/ml) or OVA (10 μg/ml). CTSB inhibitor (1 nM) was added during processing of OVA protein antigen. After co-culture for 4 days, T cells were stimulated with PMA (100 ng/ml)/ionomycin (1 μg/ml) for 6 hours. BFA (20 μg/ml) was added during the last 4 hours of stimulation. Cytokine-positive cells were identified by flow cytometry and frequency was calculated and presented in the graph. Each dot represents an individual experiment and the bar represents the mean ± SEM (n=4).

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