Supplementary Figure 3: ISH and histological analysis of epididymis from mice treated with EDE1-B10.

Eight- to nine- week old male WT mice were treated with isotype control or EDE1-B10 mAb at day +1 (n=6 mice), day +3 (n=4 mice), day +5 (n=8 mice) after infection, as described in Figure 2. a. RNA in situ hybridization (ISH) staining of epididymis at day +21 using ZIKV-specific RNA probes. Low power (scale bar = 500 μm) and high power (scale bar = 20 μm) images are presented in sequence. The images in the panels are representative of sections from 4 to 6 mice. b. H & E staining of epididymis. Low power (scale bar = 500 μm) and high power (scale bar = 20 μm) images are shown in sequence. The images are representative of sections from 3 to 5 mice.