Supplementary Figure 7: Details of the transcriptomics analysis of AMs. | Nature Immunology

Supplementary Figure 7: Details of the transcriptomics analysis of AMs.

From: A gammaherpesvirus provides protection against allergic asthma by inducing the replacement of resident alveolar macrophages with regulatory monocytes

Supplementary Figure 7

(a) Sequence and mapping statistics for raw Illumina data. (b) Validation of sample purity by assessing the expression of lineage-restricted marker genes for potential contaminants; eosinophils, neutrophils, B and T cells. (c) Unsupervised, hierarchical clustering of individual lanes demonstrating discrete clustering of biologic replicates. (d) Summary of differentially expressed (DE) genes (P<1e-5) in each pairwise comparison showing DE genes in blue in volcano plot, showing the total number of DE genes outside the bidirectional arrows, and showing in the arrowheads the direction of upregulated expression for all, moderately (log 2-fold change ± 2-4) and highly (log 2-fold change > 4) DE genes.

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