Figure 1: Upregulation of both Bcl6 and Bcl6b in antigen-specific TFH cells analyzed immediately after isolation. | Nature Immunology

Figure 1: Upregulation of both Bcl6 and Bcl6b in antigen-specific TFH cells analyzed immediately after isolation.

From: Addendum: The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding

Figure 1

Quantitative PCR analysis of Bcl6 mRNA and Bcl6b mRNA in naive helper T cells (Va11+Vb3+CD44loCD62Lhi) or PCC-specific TFH cells (PI−B220− CD11b−CD8−Va11+Vb3+CD44hiCD62LloCXCR5hi) sorted from draining lymph nodes on day 7 after subcutaneous priming with PCC in Ribi adjuvant (2 × 103 cells), followed by cDNA synthesis and amplification with Platinum SYBR Green (details as for Figs. 1–3 in the original study (Nat. Immunol. 10, 375–384 (2009))); results are presented in arbitrary units (AU) relative to the expression of β2-microglobulin mRNA, set as 1. Other antigen-specific helper T cell subsets are sorted on the basis of differences in the expression of CD62L and CXCR5 as described in detail in the original study (Nat. Immunol. 10, 375–384 (2009)).

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