Table 1 Addendum Table 1 Induction and inhibition of Bcl6 or Bcl6b in TFH cells

From: Addendum: The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding

Figure

Amplification method

Tm

Gene amplified

4c

IVT

86.88–87.32

Bcl6b

5b

None

87.02–87.31

Bcl6b

5c

STA

87.03–87.47

Bcl6b

5d

STA

82.75–83.06

Bcl6

7i

STA

82.99–83.56

Bcl6

  1. Induction of Bcl6 or Bcl6b in TFH cells selectively by DC antigen presentation and inhibition of Bcl6 by PC antigen presentation, assessed by two rounds of in vitro amplification (IVT) of RNA with a MessengerAmp II aRNA Amplification kit (Ambion), similar to what is done before microarray analysis, followed by the generation of cDNA from 10 μg amplified RNA as the starting material for SYBR Green–based quantitative PCR analysis; by specific target amplification (STA) with specific primers to generate cDNA , followed by 14 cycles of PCR preamplification and dilution of the product 1:5 for use as the starting material for SYBR Green–based quantitative PCR analysis; or the melting curve (Tm) peak signal after SYBR Green–based quantitative PCR analysis.
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