Figure 2: Primers for Bcl6 and Bcl6b amplify the expected PCR products from naive helper T cells and TFH cells. | Nature Immunology

Figure 2: Primers for Bcl6 and Bcl6b amplify the expected PCR products from naive helper T cells and TFH cells.

From: Addendum: Plasma cells negatively regulate the follicular helper T cell program

Figure 2

PCR analysis of Bcl6 and Bcl6b in naive helper T cells or antigen-specific TFH cells (5 × 103) sorted (as described in Addendum Fig. 1) on day 8 of the memory response to PCC; cDNA synthesized from RNA by random-hexamer priming was analyzed by 40 cycles of PCR with both sets of primers (Addendum Fig. 1), then primers were removed and PCR products were analyzed directly with a BigDye Terminator Cycle Sequencing kit on a 3130 Genetic Analyzer.

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