Figure 4: Selective upregulation of Bcl6 and Bcl6b in antigen-specific TFH cells with the use of DCs but not PCs for antigen presentation in vitro. | Nature Immunology

Figure 4: Selective upregulation of Bcl6 and Bcl6b in antigen-specific TFH cells with the use of DCs but not PCs for antigen presentation in vitro.

From: Addendum: Plasma cells negatively regulate the follicular helper T cell program

Figure 4

Quantitative PCR analysis of Bcl6 and Bcl6b in cells derived from CD11c+ DCs or IgM−CD138+ plasma cells (PC) sorted ex vivo with protein antigen and cultured for 4 d in vitro together with cytosolic dye CFSE–labeled naive CD4+ PCC-specific 5C.C7 αβTCR–transgenic helper T cells, followed by sorting of CD44hi 5C.C7 αβTCR–transgenic helper T cells that diluted CFSE, for RNA extraction, cDNA synthesis and SYBR Green–based quantitative PCR amplification of Bcl6 and Bcl6b (details as for Fig. 4 in the original study (Nat. Immunol. 11, 1110–1118 (2010))); results are presented in arbitrary units relative to β2-microglobulin mRNA expression.

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