Figure 5: Immunofluorescence analysis to determine the cellular localization of Nm23-H1 and EBNA-3C. | Nature Medicine

Figure 5: Immunofluorescence analysis to determine the cellular localization of Nm23-H1 and EBNA-3C.

From: Epstein-Barr virus nuclear protein EBNA-3C interacts with the human metastatic suppressor Nm23-H1: A molecular link to cancer metastasis

Figure 5

a, BJAB cells transfected were fixed and incubated with primary antibodies against Nm23-H1 (rabbit anti-NM23-H1) and EBNA-3C (A10 monoclonal), as indicated. Localization of the EBNA-3C and Nm23-H1 proteins are shown for BJAB cells transfected with and without EBNA-3C (a, left and middle panels). Colocalization of the proteins was demonstrated by merging the left and middle panels and presented as panels on the right. b, Localization of Nm23-H1 in the nucleus was quantified by counting immunopositive cells with predominantly nuclear staining in each transfected sample from 5 sets of 200 cells per field.

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