Figure 3: Comparison of manual-gate consensus and ensemble clustering results.
From: Critical assessment of automated flow cytometry data analysis techniques
Dots are color-coded by population membership as determined by ensemble clustering, with donor-derived (CD45.2+) granulocytes/monocytes in green and donor-derived lymphocytes in red. Colored polygons enclose regions corresponding to the consensus clustering of manual gates. Fluorochromes used: FITC, fluorescein isothiocyanate; PE, phycoerythrin; APC, allophycocyanin. (a,b) Sample for which all of the cell populations have been accurately identified. (c,d) Sample in which the tail of the blue population has been misclassified as orange by the algorithms, resulting in a lower F-measure for the blue population. The red, blue, green, purple and orange cell populations match cell population 1–5 of Figure 2, respectively.
