Figure 1: Summary of nucleotide-level performance for the methods evaluated.
From: Assessment of transcript reconstruction methods for RNA-seq
The plots show performance at detecting exonic nucleotides. Sensitivity (blue) indicates the proportion of known exon sequence in each genome covered by assembled transcripts, and precision (orange) indicates the proportion of reported expressed sequence confined to known exons. Some protocol variants considered all expressed transcripts (all) or excluded those of low abundance (high). Programs run with gene annotation are grouped separately. iReckon was run with complete reference annotation (full) and with transcript boundaries only (ends). Transcript reconstruction methods are described in the Supplementary Note.
