From single-molecule functional studies to atomic-resolution structures, a windfall of data sheds light on the Cas9 mechanism of targeted DNA scission.
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References
Jinek, M. et al. Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science 10.1126/science.1247997 (6 February 2014).
Nishimasu, H. et al. Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell 156, 935–949 (2014).
Sternberg, S.H. et al. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature 507, 62–67 (2014).
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Krasteva, P. CRISPR snapshots of a gene-editing tool. Nat Methods 11, 365 (2014). https://doi.org/10.1038/nmeth.2912
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DOI: https://doi.org/10.1038/nmeth.2912
